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Nortis is commercializing a new generation of in vitro systems for advancing research and drug development. The disposable chips and supporting perfusion hardware allow users to tissue engineer complex microenvironments of small units of human tissues and organs.  

 

Flexible Design for Tissue-Engineering Complex Microenvironments

Nortis is commercializing disposable microfluidic chips and supporting perfusion hardware. The chips are about half the size of a credit card. Inside the chips, one can grow vascularized tissue-microenvironments by seeding cells into tubular voids within extracellular matrix gels. The chip design offers customers the flexibility to generate a variety of tissue architectures, based on the cell types and matrix materials the user chooses.

Independent Perfusion of Separate Biological Compartments

A key advantage over currently offered 3D in-vitro models is the ability to subject the tissues to fluid flow. This flow supports tissue growth and development and allows test compounds to be administered via the perfusate. In addition to lumenal perfusion, the extralumenal tissue compartment can be perfused and used to create gradients of growth factors or other physico-chemical elements.  Importantly, these biological compartments are created by the cells or biological matrix, such that cells/tissues do not contact any artificial scaffolds or materials.

Portable, Scales, and Easy to Use

In addition to the chips, Nortis provides perfusion hardware in the form of small, portable units that contain media reservoirs and waste collection tubes. Each perfusion platform can support 3 chips and can easily be moved between the cell culture incubator, laminar flow hood, and microscope stage. Fluid flow is driven with pneumatic pressure.

Functional Analysis

The chips can be imaged using standard brightfield or fluorescence microscopy in real-time or post fixation. For endpoint analysis, such as histology, the tissues can be retrieved from the chips. Alternatively, the tissues can be digested in the chip, and the resulting cell-suspensions extracted for molecular analysis, flow cytometry, or subsequent cell culture. The perfusion fluids can also be sampled upstream or downstream of the tissue to measure metabolic end products or release of cytokines.

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